Hyaluronidase: correlation between biological assay and other methods of assay
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چکیده
منابع مشابه
Hyaluronidase: correlation between biological assay and other methods of assay.
Different workers have at various times sought to correlate the in vivo and in vitro activities of hyaluronidase preparations. Chain & Duthie (1940), drawing attention to the association between diffusion in the tissues and the mucolytic activity of testicular extracts, pointed out that the viscosityreducing and diffusing activities of various enzyme preparations were in good agreement. McClean...
متن کاملCorrelation of reductimetric and turbidimetric methods for hyaluronidase assay.
When hyaluronidases act on isolated (as distinct from native) sodium hyaluronate, three changes in the properties of the substrate solution can be measured: (1) the decrease in viscosity, (2) the loss of ability to form insoluble protein salts on acidification, (3) the increase in reducing sugar content.’ Each of these changes is the basis for a quantitative method of measurement of enzyme acti...
متن کاملHyaluronidase in ocular tissue. I. Sensitive biological assay for small concentrations of hyaluronidase.
(1) The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin (Duran-Reynals, 1929). These substances consist partly of hyaluronic acid. Hyaluronidase increases the permeability of the tissues, and this involves an increased spreading capacity of liquids and corpuscular substances. This test is not sp...
متن کاملHyaluronidase in Ocular Tissues* 1. Sensitive Biological Assay for Small Concentrations of Hyaluronidase
(1) The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin (Duran-Reynals, 1929). These substances consist partly of hyaluronic acid. Hyaluronidase increases the permeability of the tissues, and this involves an increased spreading capacity of liquids and corpuscular substances. This test is not sp...
متن کاملFactors affecting the assay of hyaluronidase.
A number of different types of assay methods have been developed for the determination of hyaluronidase activity in vitro. These include the mucin clot test (1, 2), the viscosimetric assay (3-12), the turbidimetric assay (7, 13-17), and the reductimetric assay (18). Of these, the turbidimetric and viscosimetric assays have been used most widely. In the work reported here a study has been made o...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1953
ISSN: 0306-3283
DOI: 10.1042/bj0530059